Main 'characters': Transcription factors, promoter region (TATA box), polymerase 2
-Transcription Factors (TFs) bind to the promoter region (eukaryotes need other proteins to recruit polymerase)
-TATA box (found upstream) is the site to bind for TFs
-Once binding is complete, poymerase 2 begins to read TF and transcribes on a complementary strand
Elongation:
Main 'characters': Polymerase 2, coding strand, template strand
-Elongation in transcription also occurs in a 5' to 3' direction
-The strand that is very similar to the RNA transcript is the coding/sense strand (the opposite is the template/antisense strand)
-RNA is anti parallel to the templat/antisense strand
-During transcription, Thyamine (T) is replaced by Uracil (U) (Love U and not T(ea))
Termination:
Main 'characters': AAAUAAA, intron, exon, G-cap and poly-a tail
-Transcription stops when polymerase 2 reaches the terminator AAAUAAA(downstream)
-pre mRNA can't be sent into the cytoplasm (water=oxygen filled; oxygen=bully)
-G-cap and poly A-tail put on ends of the strands to protect from bullies
-intron=junk DNA to be cut out
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